I'll just share a story with you. Excel 2010 will not even attempt to load files larger than 80 MB. Remember that Excel 2010 saves files in OpenXML by default, but if your speedsheet contains too many formulas, MS advises that you save the file as an Excel Binary Workbook (.xlsb). Check the file's size in light of the number of columns; if it's little, there shouldn't be any issues.
I hope this helps you.